ABSTRACT
In vitro embryos production from prepubertal heifers can help contribute to breeding programs; however, strategies are necessary to increase their embryo production. The aim of this study was to investigate the effects of two nutritional plans on oocyte recovery, embryo production and growth performance of prepubertal Nelore heifers. Thirty-four Nelore heifers with age of 6.5 months were divided into two feeding treatments (NP1 and NP2). The NP1 diets served as the control and NP2 diets were formulated to contain an average of 1.22-fold more energy than NP1. After 3 months of supplementation, the animals underwent follicular aspiration (ovum pick-up, OPU) every 21 d for 3 months and embryos were produced in vitro. Wither height, chest depth, body weight and subcutaneous fat of animals were measured. The number of retrieved and viable oocytes per OPU were 1.49-fold and 1.42-fold greater in NP2 heifers (p = 0.018 and p = 0.049, respectively) than those in NP1 heifers. Heifers administered NP2 produced 29.7% blastocysts, a percentage higher than NP1 animals that produced 24.40% embryos (p < 0.05). Consequently, females in the NP2 treatment showed improved body development. These results indicate a positive effect of a higher energy diet on assisted reproduction and body development in prepubertal heifers.
Subject(s)
Fertilization in Vitro , Ovarian Follicle , Cattle , Animals , Female , Fertilization in Vitro/veterinary , Embryo Culture Techniques/veterinary , Oocytes , Dietary SupplementsABSTRACT
A grande quantidade de grãos quebrados resultante do beneficiamento do arroz e seu baixo valor comercial tem levado as indústrias a buscarem alternativas para agregar valor a esse subproduto. O objetivo deste estudo foi avaliar as mudanças químicas e as propriedades viscoamilográficas em farinhas de arroz, obtidas a partir de grãos quebrados dos genótipos Irga 417, BRS Primavera e CNA 8502, submetidas ao processo de extrusão. Utilizou-se delineamento experimental inteiramente ao acaso, tipo fatorial 3x2, com quatro repetições originais e análise de variância para avaliar os dados obtidos. As farinhas dos diferentes materiais genéticos diferiram quanto ao valor nutricional e tecnológico. A farinha nativa da linhagem CNA 8502 apresentou maior teor de lipídios (0,45g 100g-1) e proteínas (10,23g 100g-1). De forma geral, as farinhas da Irga 417 apresentaram maiores teores de cinzas (0,39g 100g-1) e amilose (26,44g 100g-1). A extrusão diminuiu os teores de umidade e lipídios, elevou o teor de amilose, não afetou os teores de cinzas e proteínas e reduziu os valores de pico de viscosidade, viscosidade final, quebra de viscosidade e tendência à retrogradação, porém a magnitude da resposta dependeu das características inerentes a cada genótipo. As farinhas extrusadas apresentam potencial para aplicação em produtos instantâneos ou como ingrediente para produtos alimentícios nos quais são exigidas menores viscosidades, quando servidos a quente ou a frio, que suportem a agitação e retrogradem menos quando sejam resfriados.
The large amount of broken grains resulted from rice processing and its low commercial value have led industries to find alternatives to add value to this by product. The objective of this study was to evaluate the chemical and viscolamylographic properties changes in rice flour made from broken grains of genotypes IRGA 417, BRS Primavera and CNA 8502, subject to the extrusion process. It was used a randomized experimental design, type 3x2 factorial with four replications, and variance analysis to evaluate the data obtained for this. The flours of different genotypes differed on the nutritional value and technology. Native flour of CNA 8502 had a higher lipid content (0.45g 100g-1) and protein (10.23g 100g-1). In a general way, the flour of Irga 417 showed higher levels of ash (0.39g 100g-1) and amylose (26.44g 100g-1). Extrusion decreased the levels of moisture and lipids, increased the amylose content, didn't affect the ash and protein contents and reduced the viscosity peak, final viscosity, breakdown viscosity and retrogradation tendency, but the magnitude of the response depended of the defining features inherent genotypes. The extruded flours have potential for use in instant products or as an ingredient for food products in which lower viscosities are required when served hot or cold, supporting the agitation and retrograde least when they are cooled.
